Small Molecule Biomarker Core (SMBC) | University of Pittsburgh - The University of Pittsburgh is among the nation's most distinguished comprehensive universities, with a wide variety of high-quality programs in both the arts and sciences and professional fields.

  • http://pre.biomarkers.pitt.edu/contact-us Contact Us | Small Molecule Biomarker Core (SMBC) | University of Pittsburgh - Small Molecule Biomarker Core Address Small Molecule Biomarker Core (SMBC) 210 Salk Pavilion 335 Sutherland Dr. Pittsburgh, PA 15261 (The Pavilion on 335 Sutherland Drive is a new facility which opened in June, 2015. It is located behind and is connected to Salk Hall, 3501 Terrace Street, Pittsburgh PA 15261.)
  • http://pre.biomarkers.pitt.edu/arachidonic-acid-panel-1 Arachidonic Acid Panel - 1 | Small Molecule Biomarker Core (SMBC) | University of Pittsburgh - Cytochrome P450 and lipoxygenase pathways Method validated for several of the mono- and di-oxygenated metabolites in plasma and cerebrospinal fluid Biomarkers: Hydroxyeicosatetraenoic acid (HETE) metabolites 12-HETE 15-HETE 20-HETE Epoxyeicosatrienoic acid (EET) metabolites 8,9 - EET 11,12 - EET 14,15 - EET Dihydroxyeicosatrienoic acid (DiHETrE) metabolites
  • http://pre.biomarkers.pitt.edu/arachidonic-acid-panel-2 Arachidonic Acid Panel - 2 | Small Molecule Biomarker Core (SMBC) | University of Pittsburgh - Cyclooxygenase pathways: selected metabolites   Prostaglandin A2 (PGA2)   Prostaglandin D2 (PGD2) 15-deoxy PGD2   Prostaglandin E2 (PGE2)   Prostaglandin F1alpha (PGF1alpha) 6-keto PGF1alpha   Prostaglandin F2alpha (PGF2alpha) 11-beta PGF2alpha   Prostaglandin J2 (PGJ2)
  • http://pre.biomarkers.pitt.edu/endocrine-panel Endocrine Panel | Small Molecule Biomarker Core (SMBC) | University of Pittsburgh - Estrogens:    Estradiol LLOQ 1 pg/ml  Estrone LLOQ 1 pg/ml  Ethinylestradiol LLOQ 2.5 pg/ml  Lower limit of quantitation (LLOQ) for 0.5 ml serum/plasma Androgens:    Testosterone LLOQ 25 pg/ml  Androstenedione LLOQ 25 pg/ml  Lower limit of quantitation (LLOQ) for 0.5 ml serum/plasma  Dihydrotestosterone and DHEA under development Progestins:   
  • http://pre.biomarkers.pitt.edu/nicotinecotinine Nicotine/Cotinine | Small Molecule Biomarker Core (SMBC) | University of Pittsburgh - The procedure is designed to analyze both biomarkers of nicotine exposure in blood. Sample volume: 0.5 ml serum or plasma. Limits of Quantitation: 1 ng/ml (nicotine), 5 ng/ml (cotinine).
  • http://pre.biomarkers.pitt.edu/vitamin-d-metabolite-panel Vitamin D Metabolite Panel | Small Molecule Biomarker Core (SMBC) | University of Pittsburgh - This procedure is designed to analyze 25-hydroxy-Vitamin D2 and 25-hydroxy-Vitamin D3 in blood.Sample volume: 0.5 ml serum (preferred) or plasma Limits of Quantitation:                 25-hydroxy-Vitamin D2        2 ng/ml                  25-hydroxy-Vitamin D3        2.5 ng/ml 
  • http://pre.biomarkers.pitt.edu/n-acetylaspartate-analysis N-acetylaspartate Analysis | Small Molecule Biomarker Core (SMBC) | University of Pittsburgh - Method is validated for quantitation of N-acetylaspartate in cerebrospinal fluid.Sample volume: 20 μlLower limit of quantitation: 0.5 μM
  • http://pre.biomarkers.pitt.edu/drug-analysis Drug Analysis | Small Molecule Biomarker Core (SMBC) | University of Pittsburgh - Cytochrome P450 substrates         Injected separately or as a cocktail to assess CYP450 isoform activities         Drug         CYP450                           Caffeine         CYP1A2         Chlorzoxazone         CYP2E1         Dextromethorphan         CYP2D6         Diclofenac         CYP2C9         Esomeprazole         CYP2C19         Metoprolol         CYP2D6         Midazolam         CYP3A4         Theophylline         CYP1A2         Tolbutamide         CYP2C9
  • http://pre.biomarkers.pitt.edu/content/new-progestin-panel New Progestin Panel | Small Molecule Biomarker Core (SMBC) | University of Pittsburgh - Includes progesterone and several progestins used in common contraceptive products: etonogestrel, levonorgestrel, medroxyprogesterone acetate, and norethindrone. All have a lower limit of quantitation of 25 pg/ml for a 0.5 ml seruml/plasma sample volume.
  • http://pre.biomarkers.pitt.edu/content/estradiol-analysis-liquid-chromatographytandem-mass-spectrometry-uplc-msms Estradiol Analysis by Liquid Chromatography/Tandem Mass Spectrometry (UPLC-MS/MS) | Small Molecule Biomarker Core (SMBC) | University of Pittsburgh - The estrogen panel has been expanded to include ethinylestradiol in addition to estradiol and estrone.  PDF

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